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5. Research Background:
A. Topics of current research at zhejiangUniversity
1) Cloning and characterisation of GIS sub-family gene in Arabidopsis
2) Defining the direct target of GIS gene in Arabidopsis
3) Functional verification of seed germination mutant in Arabidopsis
4) Cloning and functional verification of Arabidopsis ANR1 homologous gene in rice
5) Cloning and functional verification of Arabidopsis GIS homologous gene in Tobacoo
B. Post-doctoral research at University of York
Since December of, 2006, I have been working as a senior Research Fellow at Prof. Ian Graham’s lab, Department of Biology, University of York. I worked on defining the role of PIF3-like bHLH transcription factors in the integration of light and cold signalling in Arabidopsis. This objectives of this project was to: 1. To define the precise role of SPT and PIL5 in mediating GA biosynthetic gene expression during seed germination, 2. To identify additional gene targets of SPT and PIL5 action through an important early developmental window: germination through seedling establishment; 3. To identify SPT and PIL5 protein interaction partners and4. To determine if SPT signals through DELLAs
From November of 2003 to November of 2006, I had workedas a Post-doctoral Research Fellow at Prof. Pierre Broun’s lab, Department of Biology, University of York. I had worked on a novel C2H2 transcription factor, GIS, which regulates several aspects of shoot maturation in Arabidopsis. we found that GIS plays a central role in the control of trichome initiation during inflorescence development and epidermal differentiation during the vegetative phase. Through a series of molecular genetic studies, we eventually elucidated that GIS acts downstream of the GA signalling pathway and controls epidermal differentiation by modulating the activity of one or more cognate regulators of trichome initiation. The novel function of GIS reveals a new mechanism of shoot maturation, representing a significant advance on our understanding of plant development. These results were published in the leading journal “Plant Cell”. Shortly after this work, we found out that GIS, together with other two C2H2 transcription factors ZFP8 and GIS2, could mediate the integration of cytokinin and gibberellin signalling in the regulation of epidermal cell fate. This finding, published in another leading journal “Development”, demonstrates that functional specialization within a transcription factor gene family can facilitate the integration of different developmental cues in the regulation of plant cell differentiation. Recently, we just published another work in “Plant Physiology” showing that the DELLA repressors in the GA signalling pathway are involved in the trichome development in Arabidopsis.
C. Previous Post-doctoral Research in LancasterUniversity of UK:
From November of 2000 to October 31, 2003, I was working as a research associate at the Department of Biological Sciences, LancasterUniversity under the supervision of Prof. Brian Forde. I worked on two research projects. The objective of my first project was to modify root architecture by manipulating ANR1 (the first reported nitrate-inducible gene) expression in roots by using GAL4 enhancer trap lines in Arabidopsis. We fused ANR1 with UAS (GAL4 upstream activation sequence) together with the additional reporter gene GUS, which was control by the 35S promoter. The objective of my second project was to identify and characterize new MADS-box genes that are nutrient regulated, in particular by N, P and S. MADS-box genes are involved in the regulation of various aspects of plant development such as root growth.The ANR1 MADS-box gene is a nitrate-inducible regulator of lateral root growth in Arabidopsis. The initial part of this project was to identify if any of these ANR1-related MADS-box genes were nutritionally regulated by Northern hybridization and real-time PCR. The noval MADS-box genes that we had studied were AGL 12, 14, 16-21, 26, 56 and 79 and ANR1. We had determined which of the above genes was nitrate-, phosphate- or sulfate-inducible (paper published in Planta).
D. MSc and PhD studies
From April 1991 to October 1993, I had studied for a MSc degree under the supervision of Prof. Benjavan Rerkasem in Chiang Mai University, Thailand. My research project examined the N fertilizer management in vegetable soybean. More than 3 papers have been published from this study. I completed my PhD in Plant Biology under the supervision of Prof. Ineke Stulen, Prof. Pieter J. C. Kuiper, (Laboratory of Plant Physiology, University of Groningen, The Netherlands) and Prof. Herman van Keulen (Plant Research International,P.O. Box 16 6700 AA Wageningen,The Netherlands). The main objectives for this project were to study the effect of N and P nutrient on grain yield, nodulation and nitrogen fixation in different soybean and vegetable soybean genotypes in both controlled environments (green-house and controlled climate growth chamber) and field. More than 6 papers have been published from this study.
6. Membership in academic societies:
Member of Assocation of Applied Biologists of UK
7. Awards and Honorary notification received:
1) As a member of staff in CNAP which has won the 2005 Queens Anniversary Prize for Higher and Further Education in UK.
2) Biographical profile was selected and published on 7th(2003-2004) Edition of Who’s Who in Sciences and Engineering. MARQUIS WHO’S WHO 121 Chanlon Road, P.O.Box 39, New Providence NJ07974-0039 USA.
3) Outstanding youth researcher for 1-2-3 outstanding researcher engineering of HubeiAcademy of Agricultural Sciences in 1999.
4) An award of the Excellent Researcher of Hubei Academy of Agricultural Sciences in 1994.
5) A PhD fellowship from WOTRO of The Netherlands in 1994.
6) An MSc degree study fellowship from Ford Foundation of US in 1993.
8. Other duties
Editor of Annals of Applied Biology (2009-2014)
9. Publications:
1) Gan, YB., Yu, H., Peng J and Broun., P. 2007. Genetic and molecular analysis of the role played by DELLA proteins in the control of trichome development in Arabidopsi.. Plant Physiology 145: 1031-1042. (SCI: IF:6.4).
2) Gan, YB., Liu, C., Yu, H., and Broun., P. 2007. Integration of cytokinin and gibberellin signalling by Arabidopsis transcription factor GIS, ZFP8 and GIS2 in the regulation of epidermal cell fate. Development 134: 2073-2081 (SCI:IF:7.3).`
(这篇论文的专题介绍:Introudction for this paper: Plant hormones get the GISt. Development 2007, 134: 1105).
3) Gan, YB., Kumimoto, R., Liu, C., Ratcliffe., O., Yu, H., and Broun., P. 2006. GLABROUS INFLORESCENCE STEMS modulates gibberellin signalling in the regulation of epidermal differentiation and shoot maturation in Arabidopsis. The Plant Cell 18:1383-1395. (SCI: IF:11.1)
4) Walch-Liu, P., Ivanov., II., Filleur, S., Gan, YB, Remans., T.,and Forde, BG. 2006. Nitrogen regulation of root branching. Annals of Botany 97:875-881. (SCI:IF:2.7)
5) Gan, YB.,, Filleur, S., Rahman, A., Gottensparre, S., and Forde BG.2005. Nutritional regulation of type I and type II MADS-box genes in Arabidopsis roots. Planta 222: 730-742. (SCI:IF:3.1)
6) Filleur, S., Walch-Liu, P., Gan, Y and Forde, BG. 2005. Nitrate and glutamate sensing by plant roots. Biochemical Society Transactions 33: 283-286. (SCI:IF:3.1)
7) Walch-Liu, P., Filleur, S., Gan, YB and Forde, BG. 2005. Signaling mechanisms integrating root and shoot responses to changes in the nitrogen supply. Photosynthesis Research 83: 239-250. (SCI:IF:2.3)
8) Walch-Liu, P, Gan, Y, Filleur, S., and Forde, BG. 2005.Nitrogen signalling and the regulation of root development. Aspects of Applied Biology 73: 99-106.
9) Gan, YB*., Stulen, I., van Keulen, H., & Kuiper, P.J.C., 2004. eD7c Spread Forex 1 Women Szh Welcome to zju biop r tD7c Spread Forex 1 Women Szh Welcome to zju bioy k k Http%3B%2F%2Fwww.400ai.com u u Webcam